Abcam, ab11419, Anti-CHOP antibody [9C8]

Size: 100µg
Anti-CHOP antibody [9C8] (ab11419) is a mouse monoclonal antibody detecting CHOP in Western Blot, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Over 230 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:9C8,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:ab11419 has been shown to recognize an epitope in the N-terminal region of DDIT3.

Product details:
Anti-CHOP antibody [9C8] (ab11419) is a House Mouse Monoclonal antibody and is validated for use in ICC/IF, WB in human samples.
Anti-CHOP antibody [9C8] (ab11419) has been cited over 237 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-CHOP antibody [9C8] (ab11419) has high sensitivity and specificity.
The specificity of Anti-CHOP antibody [9C8] (ab11419) has been confirmed by testing in knockout samples.
Anti-CHOP antibody [9C8] (ab11419) has 33 independent reviews from customers.
Anti-CHOP antibody [9C8] (ab11419) specifically detects DDIT3 (UniProt ID: P35638; Molecular weight: 19kDa) and is sold in 100 ug selling sizes.
Conjugation-ready, carrier free format available for antibody clone 9C8 -
ab233121
CHOP, also known as DDIT3, is a key protein involved in endoplasmic reticulum stress and apoptosis. The CHOP / DDIT3antibody is essential for studying its role in cellular stress responses and disease mechanisms. Accurately detecting CHOP molecular weight and investigating CHOP / DDIT3 IHC staining can provide valuable insights into conditions such as cancer, diabetes, and neurodegenerative diseases. Monitoring CHOP / DDIT3 protein levels is crucial for identifying potential therapeutic targets and understanding disease progression.
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDIT3 also known as CHOP (C/EBP homologous protein) is a transcription factor commonly expressed in cells under stress conditions such as endoplasmic reticulum stress. It has a molecular weight of approximately 29 kDa. As part of the system that manages cell stress responses DDIT3 expression can occur in various tissues but it is particularly notable in the context of cellular stress regulation. Researchers often use DDIT3 immunohistochemistry to study its presence and expression pattern in different tissues.
Biological function summary
DDIT3 plays a significant role in mediating cellular stress responses and promoting apoptosis when adaptive pathways fail. DDIT3 is not typically part of a multi-subunit complex but it acts in concert with other stress-related proteins to modulate gene expression. By inducing apoptosis DDIT3 helps remove severely damaged cells maintaining overall tissue health. However excessive DDIT3 activity under prolonged stress can lead to cell loss and tissue damage.
Pathways
DDIT3 is involved in the unfolded protein response (UPR) and endoplasmic reticulum stress pathways. It interacts with proteins such as ATF4 and PERK which are part of the mechanism that governs these pathways. DDIT3 induction contributes to the UPR pathway balancing the cell's fate between repair and apoptosis. This balance is important for cell survival under stress and avoids detrimental cellular damage.
DDIT3 has a strong connection with diabetes and neurodegenerative diseases. In diabetes DDIT3 overactivation can result in pancreatic beta-cell apoptosis worsening the disease's progression. Furthermore its involvement in neurodegenerative diseases includes its association with the accumulation of misfolded proteins exacerbating cellular stress conditions. DDIT3's interaction with proteins such as BCL2 further influences disease pathways by enhancing apoptotic signals.