Abcam, ab115072, Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric)

Size: 48Test / 96Test
Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2.
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Reacts with:Mouse, Human,
Assay type:Quantitative,
Sensitivity:>= 2 ng/well,
Range:20 - 5000 ng/well,
Assay time:2h 30m,
Assay Platform:Microplate reader

Product details:
Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular,
H3(try methyl K27)
is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.
Histone H3K27me3 is quantified colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols