Abcam, ab116193, Recombinant Human COTL1 protein

Size: 100µg
Recombinant Human COTL1 protein is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q14019,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
COTL1 also known as Coactosin-like protein is a small protein of approximately 16 kDa. This protein is expressed in a variety of tissues with notable presence in hematopoietic cells such as leukocytes. COTL1 binds actin and is involved mechanically in modulating actin polymerization. This protein also interacts with arachidonate 5-lipoxygenase (ALOX5) facilitating its enzymatic activity which plays an important role in lipid metabolism.
Biological function summary
COTL1 plays an important role in the regulation of immune responses and inflammatory processes. It acts as a cofactor stabilizing the interaction between ALOX5 and actin filaments impacting the formation of leukotrienes which mediate immune responses. COTL1 is not known to be part of a larger protein complex but it influences the function of ALOX5 suggesting a collaborative role in cellular processes related to inflammation.
Pathways
COTL1 influences the biosynthesis of leukotrienes within the arachidonic acid metabolic pathway. This pathway is significant for inflammatory response modulation. COTL1 and ALOX5 together affect the production of leukotrienes providing a connection to the eicosanoid signaling pathway. This involvement positions COTL1 as an important regulator of inflammation.
COTL1 has connections with inflammatory disorders and certain cancers. The protein's interaction with ALOX5 links it to conditions such as asthma where leukotriene pathways play a part in pathogenesis. Overexpression or dysregulation of COTL1 can also relate to specific cancers including leukemia where inflammatory signaling contributes to disease progression. Understanding the COTL1-protein interactions may offer therapeutic potential.