Abcam, ab119609, Rat TrkA ELISA Kit

Size: 1 x 96Tests
Rat TrkA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat TrkA in Rat in Cell/Tissue Extracts, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Tissue Homogenate, Cell culture supernatant, Cell Lysate,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:31.2 - 2000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's rat TrkA
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat TrkA in cell culture supernatants, cell lysates and tissue homogenates.
A TrkA specific rat polyclonal antibody from goat has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TrkA is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat TrkA amount of sample captured in plate.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TrkA also known as NTRK1 is a neurotrophin receptor and a member of the tropomyosin receptor kinase (TRK) family. It has a molecular mass of approximately 140 kDa. TrkA is expressed mostly in neuronal tissues particularly in the sensory ganglia sympathetic neurons and certain portions of the central nervous system. Mechanically TrkA activates upon binding to nerve growth factor (NGF) leading to receptor dimerization and autophosphorylation triggering downstream signaling cascades essential for neuronal growth and survival.
Biological function summary
TrkA plays an important role in the regulation of neurotrophic signaling. TrkA existing often as part of larger signaling complexes modulates neuronal differentiation development and apoptosis. It ensures proper neural connections during development by responding to NGF which is important for neurons' health and plasticity. TrkA also aids in the response to injury by promoting neuronal repair and regeneration.
Pathways
TrkA engagement activates prominent pathways like the MAPK/ERK and PI3K/AKT signaling cascades. These pathways facilitate important cellular processes like proliferation survival and metabolism. Through these pathways TrkA interacts and cooperates with proteins like SHC GRB2 and PLCγ among others to execute cellular functions. These signaling pathways enable the coordinated response needed for neural tissue maintenance and development.
TrkA abnormalities are linked to conditions like congenital insensitivity to pain with anhidrosis (CIPA) and certain cancers. Mutations in NTRK1 can disrupt NGF signaling leading to neurological deficits observed in CIPA. In cancer aberrant TrkA signaling often due to gene fusions contributes to tumorigenesis. TrkA-related signals influence oncogenic proteins such as RAS and BRAF illustrating its role in cancer progression.