Abcam, ab119857, Anti-CD86 antibody [GL-1]

Size: 100µg
Anti-CD86 antibody [GL-1] (ab119857) is a rat monoclonal antibody detecting CD86 in Flow Cytometry, IP, ICC/IF, FuncS, BL . Suitable for Mouse . - Over 30 publications
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:GL-1,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse,
Applications:IP, ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing Cd86 protein. The exact immunogen used to generate this antibody is proprietary information.P42082

Product details:
What is this antibody validated in?
Anti-CD86 antibody [GL-1] (ab119857) is a rat monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), Functional Studies, Blocking in Mouse samples.
Trusted by the scientific community
Anti-CD86 [GL-1] (ab119857) was first used in a scientific publication in 2011 and has been cited over 30 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [GL-1] also available for your convenience: ab119857, FITC -
ab234237

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis., Storage buffer-pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze|Store undiluted

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary
CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.
Pathways
CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.
CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.