Abcam, ab120129, Y-27632 dihydrochloride, Rho kinase inhibitor

Size: 1mg / 10mg / 50mg
Y-27632 dihydrochloride (ab120129) is a cell-permeable, selective Rho kinase (ROCK) inhibitor. Molecular weight (MW) 320.3, Purity >99%. - Suitable for stem cell maintenance and differentiation assays - Used as component in 3D growth matrix and brain organoid differentiation media - Available in multiple sizes to fit your experimental needs - Cited in over 125 publications
Key facts
CAS number:129830-38-2,
Purity:>99%,
Form:SolidSee storage information,
Molecular weight:320.3 Da,
Molecular formula:C14H23Cl2N3O,
PubChem:9901617,
Nature:Synthetic,
Solubility:Available in multiple formats – ab144494: Soluble in 1 of ml water to give specified mM/ml concentration; ab120129: Soluble in water to 100 mM and in DMSO to 100mM.,
Biochemical name:(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide dihydrochloride,
Biological description:Selective Rho kinase inhibitor.,
Canonical smiles:CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl,
Isomeric smiles:C[C@H](C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl,
InChi:InChI=1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11?,12?;;/m1../s1,
InChiKey:IDDDVXIUIXWAGJ-DDSAHXNVSA-N,
IUPAC Name:4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide;dihydrochloride

Product details:
Y-27632 is a research tool with a wide variety of biological effects, mainly used in the field of stem cell research for maintenance and differentiation. Commonly used as 3D growth matrix component; also a component of brain organoid differentiation media.
How others have used Y-27632 in their research:
Maintenance
of tissue culture of iPSCs; 10 μM Y-27632 for 24 hr;  Montefiori et al (2018)
Reprogramming
of breast cancer cells to induced progenitor-like cells (iPLs) ; 2 μM Y27632 ; Yuan et al (2018)
Differentiation
- Y-27632 enables human embryonic stem cells to survive and differentiate into cortical and basal telencephalic progenitors; Watanabe et al (2007)
*Also available in simple stock solutions (
ab144494
) - add 1 ml of water to get an exact, ready-to-use concentration.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Store under desiccating conditions|The product can be stored for up to 2 years

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ROCK1 and ROCK2 also known as Rho-associated coiled-coil containing protein kinases 1 and 2 are serine/threonine kinases with molecular masses of approximately 158 kDa and 160 kDa respectively. These kinases play an essential role in cell structures and functions like contraction motility and adhesion. ROCK1 and ROCK2 express in various tissues including the brain heart and skeletal muscle where they regulate actin cytoskeleton dynamics. ROCK1 and ROCK2 function as effectors for small GTPase RhoA modulating the cytoskeleton through phosphorylation of different substrates.
Biological function summary
ROCK1 and ROCK2 contribute significantly to the regulation of smooth muscle contraction actin cytoskeleton organization and cellular migration. They exist in their complex forms and primarily act by phosphorylating downstream target proteins which leads to changes in cell shape and movement. Their activity influences various cellular processes such as apoptotic regulation and tissue rigidity which are fundamental in development and homeostasis. Increased levels or activity of ROCKs link to pathological changes including vascular diseases and cancer progression.
Pathways
ROCK1 and ROCK2 are key components of the Rho/ROCK signaling pathway and the integrin-mediated signaling pathway. These pathways are vital for cellular activities like adhesion migration and proliferation. They often interact with other proteins such as LIM kinase and myosin phosphatase modulating actin-myosin contractility. The Rho/ROCK pathway in particular act as a hub for signal transduction connecting extracellular cues to intracellular responses. This pathway regulates many physiological responses by affecting various cellular substrates and signaling molecules.
ROCK1 and ROCK2 show significant involvement in cardiovascular diseases and cancer. Dysregulation of ROCK activity contributes to hypertension by affecting vascular smooth muscle contraction while aberrant ROCK signaling often results in increased cell migration and invasion in cancerous cells. In hypertension targeting ROCKs can help reduce blood pressure as ROCK inhibitors like dihydrochloride are used therapeutically. Moreover in cancer ROCK1 and ROCK2 interact with proteins such as focal adhesion kinase which lead to enhanced tumor cell migration and metastasis highlighting potential therapeutic targets.