Abcam, ab120503, BAPTA-AM, Ca2+ chelator

Size: 25mg
MW 764.7 Da, Purity >97%. Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).
Key facts
CAS number:126150-97-8,
Purity:>97%,
Form:SolidSee storage information,
Molecular weight:764.7 Da,
Molecular formula:C34H40N2O18,
PubChem:2293,
Nature:Synthetic,
Solubility:Soluble in DMSO to 100 mM,
Biochemical name:Bapta-AM,
Biological description:Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).,
Canonical smiles:CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C,
InChi:InChI=1S/C34H40N2O18/c1-23(37)47-19-51-31(41)15-35(16-32(42)52-20-48-24(2)38)27-9-5-7-11-29(27)45-13-14-46-30-12-8-6-10-28(30)36(17-33(43)53-21-49-25(3)39)18-34(44)54-22-50-26(4)40/h5-12H,13-22H2,1-4H3,
InChiKey:YJIYWYAMZFVECX-UHFFFAOYSA-N,
IUPAC Name:acetyloxymethyl 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]anilino]acetate

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Store under desiccating conditions, The product can be stored for up to 12 months

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TDP1 also known as Tyrosyl-DNA Phosphodiesterase 1 or TDP1p is an enzyme that plays an important role in DNA repair. It has a molecular mass of approximately 67 kDa and is expressed ubiquitously across various human tissues suggesting its fundamental role in maintaining genomic stability. Mechanically TDP1 acts by cleaving the phosphodiester bond between a tyrosine residue and a DNA 3' terminus which is important in resolving protein-DNA complexes that result from DNA damage.
Biological function summary
This enzyme is indispensable in the repair of topoisomerase I-mediated DNA damage participating as an important player in the DNA repair machinery. TDP1 works within the context of a complex involving other repair proteins ensuring the removal of DNA-protein cross-links that could otherwise lead to cell death if left unrepaired. This activity highlights its role in genomic stability and cell viability.
Pathways
TDP1 finds its place within the DNA single-strand break repair and the DNA damage response pathways. Its function is closely associated with other proteins such as XRCC1 which enhances the stability and efficiency of the DNA repair process and PARP1 which directs the cell's response to DNA damage. These interactions highlight TDP1's role in safeguarding genetic information by enabling precise and effective repair mechanisms.
TDP1 is notably implicated in neurodegenerative conditions such as Spinocerebellar Ataxia with Axonal Neuropathy (SCAN1) demonstrating how critical its proper function is for neuronal survival. Mutations or dysregulation in TDP1 have also been linked to heightened sensitivity to chemotherapeutic agents indicating potential challenges in cancer treatment. These disease linkages put TDP1 in association with proteins like ATM and BRCA1 which are involved in cellular responses to DNA damage and maintaining genomic integrity.