Abcam, ab121057, Anti-GLP-1 antibody [10]

Size: 100µg
Mouse Monoclonal GLP-1 antibody. Suitable for sELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GCG aa 1-50.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:10,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GCG aa 1-50. The exact immunogen used to generate this antibody is proprietary information.P01275,
Specificity:ab121057 binds the free N-terminus of GLP-1(7-37) and GLP1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and Human GIP. Cross-reacts 0.25% with GLP1(1-37), approximately 6% with Human GLP2 and approximately 50% with Exendin 4.

Product details:
Myeloma: SP2mIL6.
ab26278
should be used as capture with biotinylated version ab121057 as detection.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 2.9% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GLP-1 also known as glucagon-like peptide-1 is a 30 or 31 amino acid peptide with a mass of approximately 3.3 kDa. This peptide is mainly secreted by intestinal L-cells and the CNS. It enhances the secretion of insulin in response to glucose making it an important regulator of blood sugar levels. GLP-1 is degraded by dipeptidyl peptidase-4 which rapidly reduces its activity. Researchers often target GLP-1 in studies aimed at understanding metabolic processes and developing therapeutic interventions.
Biological function summary
GLP-1 influences several physiological functions by acting on its receptor GLP-1R a G-protein coupled receptor. It is not part of a complex but exerts its effects through binding to this receptor. Activation of GLP-1R promotes insulin secretion suppresses glucagon release slows gastric emptying and promotes satiety. This makes GLP-1 an important regulator in energy homeostasis and nutrient absorption. Its effects on appetite and food intake are of particular interest in obesity research.
Pathways
GLP-1 is an essential component of the incretin pathway which regulates insulin secretion in response to food intake. The interaction between GLP-1 and the GLP-1 receptor activates the adenylate cyclase pathway leading to increased cAMP and PKA signaling in pancreatic beta-cells. This chain of events enhances the insulin-secreting ability of these cells. Furthermore GLP-1 has connections with proteins such as insulin and glucagon through its regulatory functions in glucose metabolism.
GLP-1 is highly relevant to type 2 diabetes and obesity. Its ability to enhance insulin secretion and promote weight loss has made it a target for drug development in the treatment of these conditions. Antidiabetic medications including GLP-1 receptor agonists exploit this mechanism to control blood sugar levels effectively. Additionally GLP-1's connection to insulin makes it a significant focus in diabetes research as imbalances in these proteins contribute to the disease pathology.