Abcam, ab121250, Anti-ISY1 antibody

Size: 100µL
Rabbit Polyclonal ISY1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ISY1 aa 1-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ISY1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.Q9ULR0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ISY1 also known as Pre-mRNA-splicing factor ISY1 homolog is a component of the spliceosome machinery. It has a mass of approximately 30 kDa. This protein is expressed in various tissues including the heart brain and liver. Mechanically ISY1 participates as a splicing factor which facilitates the removal of introns from pre-mRNA. It's involved in the transition between spliceosomal complexes essential for precise and accurate splicing of mRNA.
Biological function summary
ISY1 plays a significant role in RNA splicing a process required for mRNA maturation. It is part of the spliceosome complex specifically interacting in the late stages of spliceosome activation. This interaction ensures the correct assembly of the splicing machinery which affects the processing of a wide range of mRNA precursors. By influencing the inclusion or exclusion of certain exons ISY1 affects the variety of proteins that cells can produce.
Pathways
ISY1 is involved in the RNA splicing process which is a critical component of the gene expression regulation pathway. In particular ISY1 interacts with other splicing factors like U2AF and SF3A/B during the assembly of spliceosome. It contributes to the activation of the spliceosome and progression through the splicing cycle. Any disruptions in this pathway could lead to broader consequences in gene expression affecting cellular function and homeostasis.
Alteration in ISY1 expression links to diseases like retinitis pigmentosa and certain types of cancers. Aberrant splicing in these conditions suggests a potential involvement of ISY1 in pathogenesis. Furthermore ISY1 interacts with SNRNP200 a protein implicated in retinitis pigmentosa indicating a complex network where precise splicing regulation is important for maintaining cellular health. Understanding ISY1's specific role in these disorders could provide insights for therapeutic strategies.