Abcam, ab12183, Anti-INCENP antibody

Size: 50µL
Rabbit Polyclonal INCENP antibody. Suitable for ICC, IP, WB, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Xenopus laevis, Human samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human INCENP aa 850 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Xenopus laevis,
Applications:WB, ICC, IP, IHC-Fr, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human INCENP aa 850 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q9NQS7

Product details:
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells (see image below). However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification notes-Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
INCENP short for Inner Centromere Protein plays a mechanical role during cell division. It acts as an important component of the chromosomal passenger complex (CPC) an important player in mitosis. INCENP is approximately 95 kDa in mass and is expressed largely in dividing cells. It helps to regulate chromosome alignment segregation and cytokinesis by interacting with other CPC members. Its activity is centered on the centromeres and the spindle midzone making it essential for proper mitotic progression.
Biological function summary
INCENP functions within the CPC which includes the kinase Aurora B Survivin and Borealin. This complex facilitates proper chromosomal alignment and segregation during mitosis. INCENP links Aurora B kinase activity to the centromere essential for correct chromosome bi-orientation and tension sensing. As a result INCENP stabilizes the architecture of the mitotic spindle ensuring successful cell cycle completion. This process is vital for maintaining genomic stability in proliferating cells.
Pathways
INCENP participates in the signaling cascades that control mitosis and cellular division. It is involved in the spindle assembly checkpoint pathway ensuring that cells do not progress through mitosis with misaligned chromosomes. INCENP interacts closely with proteins such as Aurora B and Survivin within this checkpoint mechanism. These interactions ensure that the cell cycle does not proceed until all chromosomes are correctly attached to the spindle apparatus thereby safeguarding against aneuploidy.
INCENP has strong connections to cancer and various chromosomal instability syndromes. Misregulation or mutation of INCENP can lead to an improper chromosomal segregation resulting in aneuploidy a hallmark of many cancer types. Its association with Aurora B and the other CPC components further implicates its role in tumorigenesis. Alterations in INCENP function could also contribute to disorders characterized by genomic instability highlighting its importance in cell division and genomic integrity.