Abcam, ab1220, Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade

Size: 100µg
Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) is a mouse monoclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ELISA, ChIP, Dot Blot . Suitable for Arabidopsis, Cow, Drosophila, Human, Rice . - Over 960 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:mAbcam 1220,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Drosophila melanogaster, Rice, Cow, Human, Arabidopsis thaliana,
Applications:ICC/IF, Dot, WB, ChIP, ELISA, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:By peptide ELISA ab1220 recognizes di methyl K9, but not unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. By Western blot ab1220 is blocked by di methyl K9, but not by unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. This indicates the specificity of ab1220 for di methyl K9 of Histone H3.

Product details:
Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) is a House Mouse Monoclonal antibody and is validated for use in ChIP, Dot, ELISA, ICC/IF, IHC-P, WB in human samples.
Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) has been cited over 961 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) has high sensitivity and specificity.
Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) has 71 independent reviews from customers.
Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) specifically detects Histone H3 Di Methyl-K9 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 ug selling sizes.
Antibody clone mAbcam 1220 is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 488, Alexa Fluor® 647 (
ab62168
ab2385
, ab23851).
Histone H3 (di methyl K9), also known as H3K9me2, is an important epigenetic modification associated with gene silencing and heterochromatin formation. This anti histone H3 antibody detecting H3K9me2 is an essential tool for studying this modification's role in transcriptional repression and chromatin structure. Abnormal H3K9me2 levels are linked to various diseases, including cancer and neurological disorders. Monitoring di methyl K9 modifications can provide valuable insights into disease mechanisms and potential therapeutic targets.
Learn about ChIP assay kits, other ChIP antibodies, and more in the
ChIP assay guide
ChIP protocols:
ChIP protocol for cross-linking ChIP (X-ChIP)
Native ChIP protocol
Chromatin preparation from tissues for ChIP
ChIP troubleshooting
ChIP tips and tricks guide
This antibody clone [mAbcam 1220] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
orders@abcam.com
or you can find further information

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle