Abcam, ab122157, Anti-RSPH4A antibody

Size: 100µL
Rabbit Polyclonal RSPH4A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RSPH4A aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RSPH4A aa 1-100. The exact immunogen used to generate this antibody is proprietary information.Q5TD94

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RSPH4A also known as radial spoke head 4 homolog A is a protein with a mass of approximately 77 kDa. It plays a mechanical role in the structure and function of cilia. RSPH4A is expressed mainly in tissues where cilia are present such as respiratory epithelium and sperm cells. The protein is an essential component of the radial spokes which are structural proteins in the axoneme of motile cilia and flagella.
Biological function summary
The proper assembly and function of cilia are contingent upon the function of RSPH4A. As part of the radial spoke complex RSPH4A interacts with other radial spoke components contributing to the regulation of ciliary and flagellar motility. This regulation is necessary for effective fluid movement across cell surfaces and for ensuring the sperm's ability to navigate toward the ovum highlighting its role in fertility.
Pathways
The integrity of cilia movement requires functional radial spoke proteins and related pathways. RSPH4A participates in the ciliary motility pathway influencing components like dynein arms and other spoke proteins such as RSPH1. These proteins collectively modulate the interaction of dynein arms with microtubules necessary for the effective beating of cilia and flagella affecting processes such as respiratory airflow and reproductive capabilities.
RSPH4A mutations have a critical link to primary ciliary dyskinesia (PCD) a condition characterized by chronic respiratory tract infections and fertility issues. RSPH4A dysfunction leads to abnormal cilia movement impacting respiratory clearance and sperm motility. Patients with PCD often exhibit disrupted coordination of cilia which can co-occur with related disorders facilitated by pathogenic variations in proteins like DNAH5 another axonemal protein.