Abcam, ab122482, Anti-CCDC65 antibody

Size: 100µL
Rabbit Polyclonal CCDC65 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC65 aa 350 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CCDC65 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8IXS2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CCDC65 also known as coiled-coil domain-containing protein 65 plays an essential role in the mechanical structure of cilia. It is a protein with a mass of approximately 58 kDa. CCDC65 is primarily found in ciliated tissues where it forms a critical part of the cilium's internal structure. The protein is associated with the axonemal organization necessary for ciliary beating and movement.
Biological function summary
This protein serves as an important component of ciliary function. CCDC65 is a part of the nexin-dynein regulatory complex which stabilizes microtubule doublets in cilia and flagella. This stabilization ensures proper bending and motility of cilia which is pivotal for cellular processes such as fluid movement and signal transduction. CCDC65 interacts with other ciliary proteins facilitating coordinated bending essential for effective ciliary movement.
Pathways
Several cellular mechanisms involve CCDC65 in the processes linked to the assembly and functioning of motile cilia. It plays an important role in the ciliary beat regulation pathway closely interacting with proteins such as GAS8 and DRC3 to maintain effective ciliary motion. These relationships enable CCDC65 to participate actively in the pathways governing cilia biogenesis and the regulation of ciliary activity.
Defects in CCDC65 are connected to primary ciliary dyskinesia (PCD) a condition characterized by impaired ciliary function leading to chronic respiratory tract infections and other respiratory issues. Research has also pointed to its association with situs inversus due to its role in ciliary motion necessary for determining left-right body asymmetry. Mutations in CCDC65 and its interaction partners GAS8 and DRC3 play a critical role in these pathological conditions highlighting its relevance in understanding genetic disorders involving ciliary dysfunction.