Abcam, ab122490, Anti-CEP295 antibody

Size: 100µL
Rabbit Polyclonal CEP295 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEP295 aa 1850-2000.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CEP295 aa 1850-2000. The exact immunogen used to generate this antibody is proprietary information.Q9C0D2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle, Store undiluted

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CEP295 also known as centrosomal protein 295 plays a critical role in centrosome function and dynamics. This protein has a molecular mass of approximately 294 kDa. CEP295 localizes at the centrosome and is heavily expressed in tissues with rapid cell division such as the brain and testes. The protein is important for proper centrosomal structure and allows for the recruitment of other centrosomal components necessary for its function.
Biological function summary
CEP295 participates in the organization and stability of centrosomes ensuring correct spindle pole formation during mitosis. The protein functions as part of a larger centrosomal complex that includes other essential structural proteins contributing to the centrosomal microtubule-nucleating activity. By facilitating centrosome cohesion CEP295 supports proper chromosome segregation and cell cycle progression.
Pathways
CEP295 engages in critical cellular processes such as the cell cycle and mitotic spindle assembly pathways. It interacts closely with proteins like PLK4 and CEP152 both involved in centrosome duplication and mitotic progression. Through these pathways CEP295 ensures that centrosomes duplicate only once per cycle maintaining genomic stability and preventing aneuploidy.
Improper functioning of CEP295 links to conditions such as microcephaly and certain cancers. The disruption of CEP295 function can lead to defective centrosomes giving rise to abnormal cell divisions that are implicated in these disorders. Proteins like CEP152 also exhibit association with microcephaly emphasizing the importance of centrosome integrity for proper neural development and cell proliferation.