Abcam, ab122901, Anti-U1-C antibody [4H12]

Size: 100µg
Rat Monoclonal U1-C antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Snrpc.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:4H12,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Mouse Snrpc.Q62241

Properties and Storage Information:
Form-Liquid, Purification notes-ab122901 was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by a propriety chromatography processes and filter sterilized., Storage buffer-pH: 6 - 8.5Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
U1-C also known as U1 small nuclear ribonucleoprotein C is a component of the U1 snRNP complex. It has a molecular mass of approximately 17 kDa and is expressed in the nucleus of eukaryotic cells. This protein plays an essential role in the spliceosome machinery which is responsible for pre-mRNA splicing. U1-C is part of a highly conserved group of proteins that engage in RNA processing and modification.
Biological function summary
U1-C interacts with U1 snRNA and other proteins to form the U1 snRNP an essential component of the spliceosome complex. The spliceosome removes introns from pre-mRNA facilitating the production of mature mRNA. U1-C stabilizes the interaction between the 5' splice site of pre-mRNA and the snRNP playing a significant part in the accuracy of splicing. Its precise function in RNA stability and processing makes it an important aspect of gene expression regulation.
Pathways
U1-C participates in the splicing pathway ensuring the correct removal of introns during RNA maturation. This protein associates closely with U1 snRNA and other spliceosomal proteins such as U1-70K. It plays a role in the regulation of alternative splicing pathways which can affect gene expression patterns. The U1-C protein indirectly influences processes like cell cycle progression and differentiation through its involvement in RNA splicing.
Malfunction or mutation of U1-C can lead to splicing abnormalities which are linked to diseases such as systemic lupus erythematosus and certain cancers. In lupus autoantibodies target the U1 snRNP complex including U1-C leading to immune system dysfunction. This association highlights the importance of U1-C in maintaining cellular and immune system homeostasis. Additionally aberrant splicing facilitated by altered U1-C function may impact oncogene or tumor suppressor pathways contributing to cancer development.