Abcam, ab124257, Anti-Pat1b antibody

Size: 100µg
Rabbit Polyclonal Pat1b antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PATL1 aa 400-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PATL1 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.Q86TB9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pat1b also known as Processing body component 4 (PATI-4) is a protein with a mass of approximately 73 kDa. It functions mechanically as an RNA-binding protein involved in mRNA decay. Pat1b enhances mRNA decay by promoting decapping and subsequently exonucleolytic degradation. It is expressed in various human tissues with prominent activity reported in the brain and testes. In molecular biology labs Pat1b is regularly studied due to its central role in mRNA turnover.
Biological function summary
The degradation of mRNA represents a critical step where Pat1b plays an essential role. As a component of the Pat1-Lsm complex Pat1b interacts with several proteins to regulate the stability and translation of mRNA. Pat1b influences the mRNA decapping process which is vital for controlled mRNA degradation thereby controlling gene expression post-transcriptionally. This process ensures the removal of aberrant or unnecessary mRNAs from the cellular environment enabling the cell to adapt to new conditions or signals rapidly.
Pathways
MRNA decay and processing pathways involve Pat1b as a central component. Within the mRNA surveillance pathway Pat1b cooperates with Dcp1-Dcp2 decapping enzymes to mediate mRNA decay. Pat1b is pivotal in the nonsense-mediated mRNA decay (NMD) pathway where it partners with Upf1 to target and degrade mRNAs containing premature stop codons. These pathways ensure cellular integrity and protein synthesis regulation.
Disruptions in Pat1b are linked to neurodegenerative diseases such as Amyotrophic lateral sclerosis (ALS) and conditions like intellectual disability. In ALS a failure in precise mRNA surveillance and decay pathways leads to toxic buildup of improperly processed mRNAs implicating Pat1b along with proteins such as TDP-43. In the context of intellectual disabilities altered mRNA metabolism involving Pat1b and its interaction with proteins like FMRP can affect neuronal function and development highlighting its significance for normal cognitive function.