Abcam, ab124372, Anti-NDP52 antibody [OTI4H5]

Size: 100µL
Mouse Monoclonal NDP52 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with African green monkey, Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CALCOCO2.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI4H5,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human, African green monkey,
Applications:WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human CALCOCO2.Q13137

Product details:
Clone OTI4H5 (formerly 4H5).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from cell culture supernatant., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDP52 also known as nuclear dot protein 52 or calcium-binding and coiled-coil domain 2 (CALCOCO2) is a selective autophagy receptor that recognizes ubiquitinated cargos. It consists of several domains that interact with both autophagic machinery and cargo. This protein has a molecular mass of approximately 52 kDa. NDP52 is expressed in various tissues with the highest levels in immune-related cells such as macrophages and dendritic cells.
Biological function summary
NDP52 plays a significant role in the clearance of intracellular pathogens and damaged organelles. As part of the autophagy process NDP52 promotes the targeting of specific ubiquitinated proteins and pathogens to the autophagosome for degradation. It operates by directly binding to LC3 proteins on the autophagosome membrane enabling the engulfment and degradation of the cargo. Additionally NDP52 is sometimes part of a complex with other autophagy receptors like p62 and optineurin enhancing its adaptability and response to cellular stress.
Pathways
NDP52 connects closely to the autophagy-lysosome pathway a critical cellular recycling process. It facilitates the degradation of intracellular bacteria by interacting with proteins like LysRS and TBK1 forming a bridge between ubiquitinated bacteria and the autophagic machinery. NDP52 also influences the NF-kB signaling pathway by modulating the turnover of NF-kB regulatory elements. Through these interactions it regulates inflammation and immune responses highlighting its importance in maintaining cellular homeostasis.
NDP52's activity relates to neurodegenerative conditions and certain infections. Its dysfunction or altered expression is implicated in the progression of disorders like Parkinson's disease where compromised autophagic clearance contributes to neuronal damage. NDP52 also links to bacterial infections such as those caused by Salmonella by mediating the host's ability to clear intracellular pathogens. Proteins like LC3 involved in autophagy and TNF receptor-associated factors in immune response further underline NDP52's role in these conditions.