Abcam, ab124604, Recombinant Mycobacterium tuberculosis TB Ag85A protein

Size: 50µg
Recombinant Mycobacterium tuberculosis TB Ag85A protein is a Mycobacterium tuberculosis H37Rv Full Length protein, in the 44 to 338 aa range, expressed in Baculovirus infected BTI-TN-5B1-4 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Baculovirus infected BTI-TN-5B1-4 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P9WQP3,
Animal free:No,
Carrier free:No,
Species:Mycobacterium tuberculosis H37Rv,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TB Ag85A also known as Antigen 85A is an important protein in Mycobacterium tuberculosis. It has a molecular mass of approximately 30-32 kDa. TB Ag85A is part of the antigen 85 complex which consists of proteins Ag85A Ag85B and Ag85C. This protein actively participates in mycobacterial cell wall synthesis. It catalyzes the transfer of mycolic acids to the cell wall arabinogalactan contributing to the bacteria's protective barrier. Expression occurs heavily in the cell envelope of Mycobacterium tuberculosis enabling vital interactions with the host's immune cells.
Biological function summary
TB Ag85A plays a significant role in the survival and virulence of Mycobacterium tuberculosis within host macrophages. It is part of the antigen 85 complex an important element in the synthesis of the mycobacterial cell envelope. This complex facilitates the interconversion of trehalose monomycolate and trehalose dimycolate critical for granuloma formation. The antigen 85 complex interferes with the host's immune response aiding the persistence of the bacterium. The synthesis and secretion of TB Ag85A by Mycobacterium tuberculosis allow it to adjust and survive hostile intra-host environments.
Pathways
TB Ag85A participates in the lipid biosynthesis pathway integral to cell envelope formation. This pathway plays a fundamental role in maintaining mycobacterial cell wall integrity. TB Ag85A is linked with proteins like Ag85B and Ag85C which are components of the same antigen 85 complex. These proteins work together within the pathway contributing to the robust defense system of Mycobacterium tuberculosis which complicates treatment and eradication efforts.
TB Ag85A is closely connected to tuberculosis a severe infectious disease. The protein's role in cell wall synthesis and immune evasion links it to the pathogen's ability to persist in the host. TB Ag85A's interactions with host immune defenses allow Mycobacterium tuberculosis to survive and proliferate in the lungs. Additionally studies propose TB Ag85A as a potential target for developing vaccines against tuberculosis given its role in infection and immune evasion. Researchers are exploring its connections to Ag85B and Ag85C for novel therapeutic interventions in tuberculosis management.