Abcam, ab124812, Anti-FADD antibody [EPR5030]

Size: 100µL / 1mL
Anti-FADD antibody [EPR5030] (ab124812) is a rabbit monoclonal antibody detecting FADD in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5030,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-FADD antibody [EPR5030] (ab124812) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.
What is the molecular weight of FADD?
Anti-FADD [EPR5030] (ab124812) specifically detects a band for FADD (UniProt: Q61160) at a molecular weight of 23kDa.
Trusted by the scientific community
Anti-FADD [EPR5030] (ab124812) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-FADD [EPR5030] (ab124812) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR5030] also available for your convenience: ab124812, PE -
ab211700
, Carrier free -
ab229444
, APC -
ab317936
, Alexa Fluor® 488 -
ab317976
, Alexa Fluor® 594 -
ab318017
, Alexa Fluor® 555 -
ab318059
, Alexa Fluor® 647 -
ab318101
, Alexa Fluor® 750 -
ab320980
Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FADD also known as Fas-Associated protein with Death Domain is an adaptor molecule with a molecular weight of approximately 23 kDa. It plays a critical role in the transmission of apoptotic signals. FADD is widely expressed in various tissues particularly in the thymus and immune system cells. This protein serves as a bridge linking death receptors like Fas and TNFR-1 with caspase activation pathways.
Biological function summary
FADD is essential in apoptosis where it assists in the assembly of the death-inducing signaling complex (DISC). Upon receptor activation FADD recruits procaspase-8 or -10 to DISC promoting their autocatalytic cleavage and activation. This leads to the subsequent cascade that results in cell apoptosis. FADD also plays a role in necroptosis and is involved in the immune response regulation highlighting its multifunctional nature in cellular processes.
Pathways
FADD integrates into the apoptotic and necroptotic pathways. In the apoptotic pathway it interacts closely with Fas a death receptor to promote caspase-8 activation. Additionally in the necroptotic pathway FADD associates with RIP1 and RIP3 contributing to an alternative form of programmed cell death. These interactions underline its significant role in controlling cell fate decisions.
Aberrations in FADD function are associated with cancer and autoimmune diseases. Overexpression or mutation of FADD can lead to unchecked cell proliferation or defective apoptosis contributing to cancer development. In autoimmune disorders improper regulation of FADD may disrupt immune tolerance and lead to systemic inflammation. Key proteins involved in these disease processes include caspase-8 and RIPK1 which interact with FADD in regulating cell death and survival mechanisms.