Abcam, ab124824, Anti-CXCR4 antibody [UMB2]

Size: 20µL / 100µL / 1mL
Anti-CXCR4 antibody [UMB2] (ab124824) is a rabbit monoclonal antibody detecting CXCR4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 230 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:UMB2,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IHC-Fr, IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognizes only the non-phosphorylated C-terminus of CXCR4 (residues 341-352). Phosphorylation of S346/347 blocks antibody binding. PMID: 24154522, 25451233.We recommend dephosphorylation of samples using lambda phosphatase treatment. Please refer to application notes.

Product details:
Product Specifications
Anti-CXCR4 antibody [UMB2] (ab124824) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB in human, mouse, rat samples.
Anti-CXCR4 antibody [UMB2] (ab124824) specifically detects CXCR4 (UniProt ID: P61073; Molecular weight: 40kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-CXCR4 antibody [UMB2] (ab124824) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CXCR4 antibody [UMB2] (ab124824) has been cited over 238 times in peer reviewed journals and is trusted by the scientific community.
Anti-CXCR4 antibody [UMB2] (ab124824) has 12 independent reviews from customers.
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ab211982
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Target Information
CXCR4 (fusin), also known as C-X-C chemokine receptor type 4 or CD184, is a protein encoded by the CXCR4 gene in humans. CXCR4 or CD184 protein is a receptor for the chemokine CXCL12 (SDF-1) and plays a significant role in oncology due to its involvement in tumor growth, metastasis, and the tumor microenvironment. CXCR4 and its ligand CXCL12 are crucial for the metastatic spread of cancer cells to organs where CXCL12 is expressed, such as the bone marrow, liver, and lungs. The CXCR4-CXCL12 axis helps tumor cells find niches that support their survival and growth.
Our internal data indicates that rat is not recommended for IHC.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (
ab205718
) and Goat Anti-Rabbit Alexa Fluor® 488 (
ab150077
). Or search our wide range of secondary antibodies for use with your experiment.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CXCR4 also known as C-X-C chemokine receptor type 4 is a G protein-coupled receptor that is involved in signal transduction. It has a molecular weight of approximately 41 kDa. CXCR4 is ubiquitously expressed across various tissues including immune cells like T and B lymphocytes as well as in bone marrow brain and heart. It binds specifically with the ligand CXCL12 also known as stromal cell-derived factor 1 (SDF-1) facilitating responses such as cell migration and proliferation.
Biological function summary
CXCR4 plays an important role in the immune system hematopoiesis and angiogenesis. It does not function alone and is often part of a larger protein complex where it recruits and activates other G proteins. The receptor mediates chemotactic responses directing cells to sites of inflammation or injury. Its interaction with CXCL12 is critical for maintaining immune surveillance aiding in the movement and positioning of immune cells.
Pathways
CXCR4 integrates into significant cellular signaling pathways such as the PI3K/AKT pathway and the MAPK pathway. It collaborates closely with signaling proteins like AKT1 and MAPK1 impacting cell survival and growth. These pathways are essential for various cellular functions including cell cycle progression and apoptosis regulation. The cross-talk between CXCR4 and these pathways underlines its influence on cell fate decisions.
CXCR4 is implicated in cancer metastasis and HIV entry into cells. Overexpression of CXCR4 is observed in several cancers contributing to tumor growth and metastasis. The interaction between CXCR4 and CXCL12 facilitates the infiltration and spread of cancer cells. Additionally in HIV CXCR4 serves as a coreceptor along with CD4 allowing the virus to enter and infect host cells. Both cancer and HIV illustrate CXCR4's central role in disease progression and pathogenesis.