Abcam, ab124920, Anti-Munc18-1 antibody [EPR4850]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Munc18-1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4850,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Munc18-1 also known as Syntaxin-binding protein 1 is an important protein involved in the regulation of neurotransmitter release at synapses. It has a molecular mass of approximately 67 kDa. This protein is highly expressed in the brain where it plays a significant role in synaptic vesicle exocytosis. Munc18-1 interacts with components of the SNARE complex facilitating the docking and fusion of synaptic vesicles with the presynaptic membrane.
Biological function summary
This protein is important for the proper release of neurotransmitters into the synaptic cleft. Munc18-1 functions as a part of the neuronal exocytosis complex where it regulates the interaction with syntaxin-1 a core SNARE protein. This protein-protein interaction stabilizes an intermediate state necessary for vesicle priming and subsequent fusion ensuring efficient synaptic transmission. By controlling vesicle movement Munc18-1 influences communication between neurons.
Pathways
Munc18-1 integrates into the neurotransmitter release pathway which is central to synaptic function. It closely associates with the SNARE complex involving proteins such as SNAP-25 and synaptobrevin. Another important pathway is the synaptic vesicle cycle where Munc18-1 plays a role in vesicle docking and priming stages. Through these interactions it ensures that synaptic vesicles are ready and able to release their contents upon stimulation.
Alterations in Munc18-1 expression or function connect to neurological conditions like epilepsy and intellectual disability. In epilepsy dysregulation of neurotransmitter release due to modified Munc18-1 function disrupts synaptic communication leading to seizure activity. In the context of these disorders Munc18-1 interacts functionally with proteins such as VAMP2 contributing to the pathological state when disrupted. These interactions highlight the importance of Munc18-1 in maintaining normal neurological function.