Abcam, ab124956, Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]

Size: 100µL / 1mL
Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956) is a rabbit monoclonal antibody detecting JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF, Dot Blot . Suitable for Human, Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 240 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5693,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:Flow Cyt (Intra), ICC/IF, IP, Dot, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody will detect will detect JNK1 (pT183), JNK2 (pT183) and JNK3 (pT221).

Product details:
What is this antibody validated in?
Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human, Mouse samples.
Trusted by the scientific community
Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) [EPR5693] (ab124956) was first used in a scientific publication in 2012 and has been cited over 240 times in peer-reviewed journals.
Reviewed by scientists
Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) [EPR5693] (ab124956) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR5693] also available for your convenience: ab124956, Alexa Fluor® 488 -
ab201862
, Alexa Fluor® 647 -
ab201864
, PE -
ab208843
, Carrier free -
ab219584
, APC -
ab310817
, Alexa Fluor® 594 -
ab311653
, Alexa Fluor® 568 -
ab312926
, Alexa Fluor® 555 -
ab313139
, Alexa Fluor® 750 -
ab320994
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
JNK1 JNK2 and JNK3 are c-Jun N-terminal kinases also known as stress-activated protein kinases (SAPKs). These kinases play significant roles in cellular stress responses. Commonly referred to by their shorter names JNK1 JNK2 and JNK3 they possess molecular weights of approximately 46 kDa to 55 kDa depending on their isoforms. These kinases are widely expressed across various tissues with JNK1 and JNK2 seen in most tissues whereas JNK3 shows more expression in neural tissues. Activation of JNKs occurs through phosphorylation at threonine 183 (T183) for JNK1 and JNK2 and threonine 221 (T221) for JNK3.
Biological function summary
C-Jun N-terminal kinases play important roles in mediating responses to stress stimuli including cytokines and ultraviolet irradiation. They are known to be part of the MAPK signaling complex and have direct involvement in the regulation of genes connected to apoptosis and cellular proliferation. JNK1 and JNK2 in particular have broad cellular roles and influence the activity of transcription factors such as c-Jun impacting gene expression significantly. JNK3 meanwhile contributes more to neuronal apoptosis given its expression pattern.
Pathways
JNK1 JNK2 and JNK3 actively engage in the MAPK signaling pathway connecting with several upstream and downstream proteins like MKK4 and MKK7 which serve as upstream kinases and c-Jun which acts downstream. The MAPK pathway is critical for translating extracellular signals into a wide range of cellular processes. The involvement of JNKs in this pathway highlights their contribution to balancing cell survival and death signals placing them alongside related proteins like ERK and p38MAPK within the signaling hierarchy.
The activation and regulation of JNK1 JNK2 and JNK3 have essential implications in neurodegenerative diseases and cancer. JNK3 in particular is associated with neurodegenerative conditions such as Alzheimer's disease due to its role in neuronal stress-induced apoptosis. Similarly aberrant JNK1 and JNK2 activation connects to various cancers through their influence on genes governing cell cycle and apoptosis often in tandem with oncogenes or tumor suppressors like p53 and Bcl-2. These associations highlight the therapeutic potential of targeting these kinases in disease treatment strategies.