Abcam, ab124964, Anti-alpha smooth muscle Actin antibody [EPR5368]

Size: 20µL / 100µL / 1mL
Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) is a rabbit monoclonal antibody detecting alpha smooth muscle Actin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 390 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5368,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) was first used in a scientific publication in 2014 and has been cited over 393 times in peer reviewed journals. It's performance in Western Blot and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) has been confirmed by Western Blot testing in alpha smooth muscle Actin knockout HeLa cells.
Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) has 20 independent reviews from customers.
alpha smooth muscle Actin antibodies are often used as loading controls in Western Blot. Anti-alpha smooth muscle Actin antibody [EPR5368] has been verified in Western Blot samples and detects a band at 42kDa Molecular weight.
Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) specifically detects alpha smooth muscle Actin (UniProt ID: P62736; Molecular weight: 42kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR5368 -
ab220795
Antibody clone EPR5368 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
555, Alexa Fluor
594, PE, APC (
ab202295
ab202296
ab202509
ab202510
ab208844
ab225143
Alpha-Smooth Muscle Actin (α-SMA) is a critical protein involved in smooth muscle contraction, encoded by the ACTA2 gene. Predominantly found in vascular smooth muscle cells, α-SMA plays a vital role in fibrogenesis and wound healing. It is often used as a marker for myofibroblast formation, which are specialized cells that contribute to tissue repair and fibrosis. The expression of α-SMA is associated with the activation of myofibroblasts, making it an important indicator in studies of fibrosis and other related conditions.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
Biological function summary
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
Pathways
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.