Abcam, ab125066, Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

Size: 100µL / 1mL
Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) is a rabbit monoclonal antibody detecting Glutathione Peroxidase 4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Clone EPNCIR144 is the most cited clone to Glutathione Peroxidase 4 - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 560 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPNCIR144,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) was first used in a scientific publication in 2012 and has been cited over 567 times in peer reviewed journals. It's performance in Western Blot and IHC in mouse and human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) has been confirmed by Western Blot testing in Glutathione Peroxidase 4 knockout HeLa cells (
ab262509
Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) has 12 independent reviews from customers.
Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) specifically detects Glutathione Peroxidase 4 (UniProt ID: O70325; Molecular weight: 22kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPNCIR144 -
ab219592
Antibody clone EPNCIR144 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
ab314333
ab314334
Top cited antibody for this target with >1000 citations. Glutathione peroxidase 4 is a master regulator of ferroptosis and lipid peroxidation. This antibody is crucial in cancer research, particularly in understanding GPX4's role in cell death and antioxidant defence and is widely used in studies of reactive oxygen species (ROS) and GPX4 inhibitors for therapeutic applications.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutathione Peroxidase 4 (GPX4) also known as GXP4 or 1B4 is an essential selenoprotein with a molecular weight of approximately 22 kDa. It acts mechanically by reducing lipid hydroperoxides to their corresponding alcohols and free hydrogen peroxide to water utilizing glutathione as a cofactor. GPX4 is ubiquitously expressed with high expression levels found in the testis indicating its importance in spermatogenesis.
Biological function summary
This enzyme protects cells from oxidative damage by maintaining cellular redox balance. GPX4 exists as a monomer and is not part of larger complexes. It enables cells to counteract the harmful effects of reactive oxygen species (ROS) safeguarding lipid membranes from peroxidation. The protective function extends to the nervous system and other tissues where oxidative stress might lead to cellular damage.
Pathways
This enzyme plays a critical role in the antioxidant defense system. It integrates with the glutathione pathway where it operates alongside glutathione reductase and glutathione synthetase to detoxify cells. The protein also interacts with lipid metabolism pathways maintaining membrane integrity and function. These pathways further link GPX4 with other oxidative stress-related proteins and cellular survival mechanisms.
GPX4 has connections to neurodegenerative diseases like Parkinson's disease and cancer. In neurodegeneration low GPX4 levels can lead to increased lipid peroxidation highlighting its protective function against neuronal damage. In cancer altered GPX4 expression associates with the oxidative stress response affecting tumor progression. The protein's interactions with key oxidative stress regulators suggest potential targets for therapeutic intervention.