Abcam, ab125148, PE Anti-Macrophage antibody [BM8]

Size: 50µg
Rat Monoclonal Macrophage antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Macrophage protein.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:BM8,
Isotype:IgG2a,
Conjugation:PE,
Excitation/Emission:Ex: 480;565nm, Em: 578nm,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2Preservative: 0.09% Sodium azideConstituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Macrophages also known as histiocytes are immune cells involved in many functions in the body. They have a mass that varies depending on their stage of activation and location. These cells are primarily expressed in tissues and organs such as the liver spleen lungs lymph nodes and bone marrow. They belong to the mononuclear phagocyte system and are key players in antigen presentation and phagocytosis. These cells can be visualized using macrophage immunohistochemistry (IHC) techniques and imaged with macrophage flow cytometry. Macrophage staining and macrophage immunofluorescence staining allow researchers to assess their presence and state.
Biological function summary
These versatile cells maintain homeostasis and play roles in tissue repair and inflammation. They are part of protective mechanisms of body engaging in destruction of pathogens and mediation of immune responses. Macrophages can release cytokines chemokines and other molecules that influence immune cell proliferation and differentiation. In mouse models macrophage markers like BM8 help to identify their populations and quantify their roles in various experiments.
Pathways
These immune cells participate in both the innate and adaptive immune responses. They influence the NF-kB and MAPK pathways both of which play significant roles in stress and inflammatory responses. Macrophages interact with proteins like Toll-like receptors (TLRs) to activate downstream signaling that leads to altered gene expression necessary for immune response. They serve as critical components in pathways that facilitate communication between different cell types in immune system.
Macrophages have strong links with chronic inflammation and autoimmune diseases such as rheumatoid arthritis. They contribute to the pathogenesis of these conditions by secreting pro-inflammatory cytokines and other mediators. A connection exists between macrophages and the dysfunction of various proteins involved in inflammatory diseases such as TNF-alpha and interleukin-1. Alterations in macrophage activation and signaling could exacerbate disease progression highlighting their importance in therapeutic strategies aimed at modulating immune responses.