Abcam, ab125247, Anti-GAPDH antibody [GA1R] - Loading Control

Size: 100µg
Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) is a mouse monoclonal antibody detecting GAPDH in Western Blot . Suitable for Chicken, Escherichia coli, Hamster, Human, Mouse, Rabbit, Rat, S.cerevisiae, Spodoptera frugiperda (SF9 cells) . - Over 130 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:GA1R,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Fall armyworm,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Rabbit GAPDH.P46406,
Specificity:ab125247 recognizes native and denatured forms of GAPDH.

Product details:
What is this antibody validated in?
Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Chicken, Escherichia coli, Hamster, Human, Mouse, Rabbit, Rat, Saccharomyces cerevisiae, Spodoptera frugiperda (SF9 cells) samples.
What is the molecular weight of GAPDH?
Anti-GAPDH [GA1R] - Loading Control (ab125247) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P04406) at a molecular weight of 36kDa.
Trusted by the scientific community
Anti-GAPDH [GA1R] - Loading Control (ab125247) was first used in a scientific publication in 2012 and has been cited over 130 times in peer-reviewed journals.
Reviewed by scientists
Anti-GAPDH [GA1R] - Loading Control (ab125247) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Protein A affinity chromatography from TCS, Storage buffer-pH: 7.2Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.