Abcam, ab125604, Recombinant human PRKX protein

Size: 10µg
Recombinant human PRKX protein is a Human Full Length protein, in the 1 to 358 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>90% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity was determined to be 150 nmol/min/mg,
Accession:P51817,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
ab204872
(PAK peptide substrate) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRKX also known as Protein Kinase X is a serine/threonine-protein kinase with a molecular mass of about 37 kDa. This protein is part of the cAMP-dependent protein kinase family. Scientists observe PRKX expression mainly in the kidney heart and central nervous system. It helps regulate multiple functions by phosphorylating various substrates which are important to cell signaling and communication. The presence of the protein in specific tissues suggests a significant role in development and organ function.
Biological function summary
The protein functions as an important regulator in cellular signaling processes. PRKX interacts with other proteins as a monomer rather than as part of a larger complex. Its activity influences cellular growth differentiation and gene expression. The kinase activity shows high involvement in developmental pathways across numerous cell types. Additionally its interactions affect cellular features like polarization and motility which are important for normal physiological processes.
Pathways
PRKX serves important roles in the PKA signaling pathway and the renal development pathway. PKA signaling involves cAMP as a second messenger and PRKX acts downstream as a phosphorylating agent for various targets within the cell. In the renal development pathway PRKX collaborates with other protein kinases like PRKY which shares a similar sequence and function reflecting redundancy and specialization in developmental roles in kidneys.
The role of PRKX becomes significant in congenital kidney disorders particularly certain types of cystic kidney diseases. The protein's influence on renal development highlights its connection to these conditions. Additionally anomalies in PRKX expression or function can link to central nervous system disorders influencing neurological development and function. Within these contexts PRKX often interacts with related protein networks involving kinases and substrates important for maintaining cellular health and functionality.