Abcam, ab125661, Recombinant human PDE1A protein

Size: 5µg / 10µg
Recombinant human PDE1A protein is a Human Full Length protein, in the 2 to 545 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab125661 was determined to be 12250 - 12290 nmol/min/mg.,
Accession:P54750,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 1.05% MOPS, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphodiesterase 1A also known as PDE1A is a protein that breaks down cyclic nucleotides like cAMP and cGMP in cells. This enzyme belongs to the PDE family which influences cyclic nucleotide signaling. PDE1A has a molecular mass of about 61 kDa. The protein expresses widely across various tissues including the brain heart and lungs. Its activity relies on calcium/calmodulin which makes it an important player in cellular processes that involve calcium-dependent signaling.
Biological function summary
PDE1A regulates the levels of cyclic nucleotides within cells impacting signaling pathways that control various physiological responses. It does not exist as part of a larger complex but it works closely with other signaling molecules. PDE1A modulates cellular responses to external signals by controlling the degradation rate of cyclic AMP and cyclic GMP influencing functions like muscle contraction and neuronal signaling.
Pathways
PDE1A plays important roles in pathways such as the cAMP signaling pathway and the calcium signaling pathway. It interacts with proteins such as Protein Kinase A (PKA) in the cAMP pathway where it fine-tunes the cellular response to external stimuli like hormones. By regulating these pathways PDE1A helps maintain cellular homeostasis and influences how cells respond in various signaling contexts.
PDE1A is linked to cardiovascular diseases and certain neurological disorders. In cardiovascular conditions abnormal PDE1A activity may contribute to uncontrolled muscle contractions and heart failure. In neurological disorders changes in PDE1A expression can disrupt normal neuronal signaling and are associated with conditions such as schizophrenia. The protein's interaction with calcium/calmodulin is critical in these conditions affecting how cells respond to signaling changes that contribute to disease development.