Abcam, ab125871, Anti-SAR1 antibody

Size: 100µL
Rabbit Polyclonal SAR1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SAR1A aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SAR1A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9NR31

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab125871 is purified by antigen-affinity chromatography., Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SAR1 also known as SAR1A or SAR1B is a small GTPase belonging to the Ras superfamily. It plays an integral role in protein transport by regulating the formation of transport vesicles from the endoplasmic reticulum (ER). SAR1 has a molecular mass of about 21 kDa. It is ubiquitously expressed with significant levels observed in tissues involved in high protein synthesis activities such as liver and pancreas. Mechanically it switches between GDP-bound inactive and GTP-bound active states which is essential for its function in vesicle formation.
Biological function summary
SAR1 is an important component in the coat protein complex II (COPII) which is vital for ER-to-Golgi transport. Within this complex SAR1 assists in the initiation of vesicle budding by recruiting other COPII components such as Sec23/Sec24 and Sec13/Sec31. This assembly is important for maintaining efficient secretory pathways and cellular homeostasis. The COPII complex with SAR1 ensures the proper sorting and packaging of proteins destined for the Golgi apparatus.
Pathways
SAR1 plays an essential role in the secretory pathway facilitating intracellular protein trafficking. This pathway is vital for transporting proteins from the ER to the Golgi impacting processes such as cell signaling and membrane composition. SAR1 interacts with other proteins including Sec23 and Sec24 to ensure proper vesicle formation and function. These interactions highlight SAR1's role within the broader exocytic pathway signifying its importance in maintaining cellular function.
SAR1 mutations have been linked to lipid absorption disorders such as chylomicron retention disease (CMRD) and Anderson's disease. These conditions result from defective protein trafficking leading to impaired chylomicron secretion. In CMRD SAR1 dysfunction affects the related APOB protein contributing to the accumulation of fat in enterocytes and subsequent clinical symptoms. Understanding SAR1's function in these diseases may offer insights into therapeutic strategies targeting protein transport mechanisms.