Abcam, ab125917, Anti-OCRL antibody

Size: 50µL
Rabbit Polyclonal OCRL antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OCRL aa 550-900.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human OCRL aa 550-900. The exact immunogen used to generate this antibody is proprietary information.Q01968

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The OCRL protein also known as oculocerebrorenal syndrome of Lowe protein is a phosphatidylinositol 45-bisphosphate 5-phosphatase involved in cellular membrane trafficking. It has a molecular mass of approximately 105 kDa. Localization of OCRL occurs in several cellular compartments including the endosomes and the Golgi apparatus. This protein is widely expressed in tissues such as the brain kidney and eyes. Scientists often study OCRL to better understand its role in cellular functions and associated disorders.
Biological function summary
OCRL participates in the regulation of phosphoinositide metabolism by dephosphorylating phosphatidylinositol 45-bisphosphate an important signaling lipid. OCRL is a member of the inositol polyphosphate 5-phosphatase family and associates with clathrin-mediated endocytosis complexes. The protein ensures proper vesicular trafficking and maintenance of cell membrane dynamics impacting processes like endocytosis and ciliogenesis. Researchers focus on understanding OCRL's interactions and dynamics to comprehend its contribution to these processes.
Pathways
OCRL plays a significant role in the phosphoinositide signaling pathway and is integral to cellular trafficking pathways. In the phosphoinositide signaling pathway OCRL modulates the availability of phosphatidylinositol 45-bisphosphate interacting with proteins like APPL1 and Rab GTPases. In cellular trafficking pathways OCRL coordinates with proteins such as clathrin and dynamin to regulate endocytosis and endosomal function. Understanding OCRL's placement within these pathways provides insight into its impact on cellular homeostasis.
OCRL mutations are linked to Lowe syndrome and Dent disease both of which involve renal and ocular complications. Lowe syndrome characterized by congenital cataracts intellectual disabilities and renal Fanconi syndrome directly associates with OCRL dysfunction. The OCRL protein interacts with clathrin and Rab GTPases contributing to the pathogenesis of these disorders by disrupting normal vesicle trafficking processes. Research on OCRL-related mechanisms in these diseases seeks to unravel potential therapeutic targets and interventions.