Abcam, ab125963, Human Complement C5 ELISA Kit

Size: 1 x 96Tests
Human Complement C5 ELISA Kit is a sandwich ELISA used to quantify Human Complement C5 with a sensitivity of 63 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Wide dynamic range – quantifies 0.156 – 10 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Saliva, Plasma, Milk, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 63 pg/mL,
Range:0.156 - 10 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Human Complement C5 ELISA Kit ab125963 is a sandwich ELISA kit to measure Human Complement C5 in Serum, Plasma, Cerebral Spinal Fluid, Saliva, and Milk with a sensitivity of 63 pg/mL.
How the assay works
Human Complement C5 ELISA Kit ab125963 uses a pair of antibodies each specific of different epitopes at the target of interest : capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay Specificity
This kit recognizes both native human Complement C5 in serum, plasma, saliva, milk and CSF samples. No significant cross-reactivity observed with human complement C1,C2, C3, C4, C6, C7, C8, C9, factor B, factor D, factor H, factor I, and factor P proteins.
Human Complement C5 ELISA Kit ab125963 protocol summary
1. Add samples and standards to wells. Incubate at room temperature
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature
3. Wash each well and add Streptavidin Solution. Incubate at room temperature
4. Add TMB Solution to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately
How other researchers are using Human Complement C5 ELISA Kit ab125963
Human Complement C5 ELISA Kit ab125963 has been used to study RNAi therapeutics targeting C5
, and the pharmacokinetics of anti-C5 antibodies
References:
L. Kusner et al., 2019, PMID: 31193726
A. Latuszek et al., 2020, PMID: 32384086
Get results in 90 minutes with our SimpleStep ELISA
- Human Complement C5 ELISA Kit
ab323604

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.
Biological function summary
The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.
Pathways
The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.
C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.