Abcam, ab126431, Phosphotyrosine In-Cell ELISA Kit

Size: 1 x 96Tests
Phosphotyrosine In-Cell ELISA Kit is a Cell-based (qualitative) ELISA for the measurement of Phosphotyrosine In-Cell in Human, Mouse, Rat in Cell Samples samples.
Key facts
Detection method:Colorimetric,
Sample types:Adherent cells,
Reacts with:Mouse, Rat, Human,
Assay type:Cell-based (qualitative),
Assay time:5h 10m,
Assay Platform:Microplate

Product details:
ab126431 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Phosphotyrosine and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. By determining Phosphotyrosine protein in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.
In the Cell-Based Phosphotyrosine ELISA kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, HRP-Anti- Phosphotyrosine is pipetted into the wells and incubated. The wells are washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.
Biological function summary
Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.
Pathways
The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.
The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.