Abcam, ab126625, Anti-p150 CAF1/CAF antibody [EPR5576(2)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal p150 CAF1/CAF antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 19 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5576(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p150 CAF1 also known as CAF protein and CAF1 protein is part of the chromatin assembly factor 1 complex. It weighs approximately 150 kDa. This protein primarily expresses in the nucleus of eukaryotic cells where it plays an important role in DNA replication and repair. The p150 protein acts as a structural component that is essential for proper chromatin assembly during the S phase of the cell cycle.
Biological function summary
This target functions as part of the CAF-1 complex which includes two additional subunits—p60 and p48. The complex aids in the assembly of newly synthesized histones onto replicating DNA facilitating nucleosome formation. It affects chromatin dynamics influencing transcriptional regulation by providing nucleosome assembly binding sites along DNA. This process maintains genomic integrity during cell division and influences gene expression in response to various cell signals.
Pathways
The p150 protein interacts within pathways involved in DNA replication and repair. It plays a major role in the nucleosome assembly pathway closely interacting with histone proteins H3 and H4. Through these interactions it ensures the stability of replication forks during DNA synthesis. Besides it participates in the regulation of the cell cycle and influences the chromatin assembly pathway important for accurate DNA replication and cell division.
Several conditions show connection with the p150 protein's function in DNA replication. Alterations or defects in its expression link to cancer where chromatin misassembly leads to genomic instability a hallmark of tumorigenesis. Additionally the involvement of p150 in neurodegenerative disorders sheds light on how disrupted chromatin assembly can affect neural cells. Its interaction with proteins like retinoblastoma (Rb) points to the potential dysregulation in cell cycle control further influencing disease progression.