Abcam, ab126729, Anti-Netrin 1 antibody [EPR5428]

Size: 20µL / 100µL / 1mL
Anti-Netrin 1 antibody [EPR5428] (ab126729) is a rabbit monoclonal antibody detecting Netrin 1 in Western Blot, IP . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5428,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Netrin 1 antibody [EPR5428] (ab126729) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.
What is the molecular weight of Netrin 1?
Anti-Netrin 1 [EPR5428] (ab126729) specifically detects a band for Netrin 1 (UniProt: O95631) at a molecular weight of 67kDa.
Trusted by the scientific community
Anti-Netrin 1 [EPR5428] (ab126729) was first used in a scientific publication in 2012 and has been cited over 40 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-Netrin 1 antibody [EPR5428] (ab126729) has been confirmed by Western blot testing in NTN1 Knockout MCF7 cell line,
ab323856
Other related products
We have a range of other formats of antibody clone [EPR5428] also available for your convenience: ab126729, Carrier free -
ab201324
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Netrin-1 also known as NTN1 or Netrin-1 protein is a guidance cue important for cell migrations. It falls under the netrin proteins and has an approximate mass of 70 kDa. This protein is mainly expressed in the central nervous system especially during development but also appears in certain developing tissues and organs. Netrin-1 interacts with various receptors influencing axon guidance and cell motility.
Biological function summary
Netrin proteins play key roles in directing neuronal and vascular development. Netrin-1 influences axon pathfinding by attracting or repelling growth cones and also participates in angiogenesis. It does not usually form a stable complex but functions through interactions with distinct receptor types like DCC Neogenin and UNC5 homologue proteins. These interactions determine the direction of cellular movement and position within tissues.
Pathways
Netrin-1 integrates within signaling pathways such as the MAPK and PI3K/AKT pathways. It influences neuron migration and blood vessel formation within these pathways. Netrin receptors like DCC and UNC5B are important in transducing netrin-1 signals. These pathways involve intricate signaling cascades modulating cell survival growth and trajectory establishing netrin-1 as significant in neurodevelopmental processes.
Researchers link netrin-1 to neurodegenerative diseases and cancer metastasis. Abnormal levels of netrin-1 can associate with disorders like Alzheimer's disease and various cancers. In cancer netrin-1 interaction with its receptors might influence tumour cell survival and invasion resembling mechanisms found in neuronal cells. Understanding the role of netrin-1 in these conditions could uncover valuable therapeutic targets potentially involving DCC and UNC5 homologues among other associated molecules.