Abcam, ab128568, Anti-CPT1A antibody [8F6AE9]

Size: 100µg
Anti-CPT1A antibody [8F6AE9] (ab128568) is a mouse monoclonal antibody detecting CPT1A in Western Blot, Flow Cytometry, IHC-P, ICC/IF, ELISA . Suitable for Human, Mouse, Rat . - Clone 8F6AE9 is the most cited clone to CPT1A - KO validated for confirmed specificity - Over 170 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:8F6AE9,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:In-Cell ELISA, ICC/IF, IHC-P, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CPT1A antibody [8F6AE9] (ab128568) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P, In-Cell ELISA, WB in human, rat samples.
Anti-CPT1A antibody [8F6AE9] (ab128568) has been cited over 179 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-CPT1A antibody [8F6AE9] (ab128568) has high sensitivity and specificity.
The specificity of Anti-CPT1A antibody [8F6AE9] (ab128568) has been confirmed by testing in knockout samples.
Anti-CPT1A antibody [8F6AE9] (ab128568) has 5 independent reviews from customers.
Anti-CPT1A antibody [8F6AE9] (ab128568) specifically detects CPT1A (UniProt ID: P50416; Molecular weight: 88kDa) and is sold in 100 ug selling sizes.
Antibody clone 8F6AE9 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, HRP (
ab171449
ab198494
CPT1A is crucial in fatty acid oxidation, and its dysfunction is linked to metabolic disorders, diabetic nephropathy, and certain cancers
This monoclonal antibody to CPT1A has been knockout validated in Western blot. The expected band for CPT1A was observed in wild type cells and the band was not seen in CPT1A knockout cells.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Precipitation Ammonium Sulphate, Purification notes-Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation., Storage buffer-pH: 7.4 - 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CPT1A also known as carnitine palmitoyltransferase 1A is a vital enzyme involved in the transport of long-chain fatty acids across the mitochondrial membrane. This protein facilitates the conversion of fatty acids into acyl-carnitine a process necessary for beta-oxidation within the mitochondria. The molecular weight of CPT1A is approximately 88 kDa. CPT1A predominantly expresses in liver cells where energy metabolism from fatty acids is important. Alternate names for this target include CPT 1 and CTP1A reflecting its central role in metabolic processes related to lipid utilization.
Biological function summary
CPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.
Pathways
CPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.
CPT1A is notably associated with metabolic syndromes and fatty liver disease. Mutations or deficiencies in CPT1A can lead to disorders like hepatic carnitine palmitoyltransferase 1A deficiency characterized by hypoketotic hypoglycemia and hepatomegaly. Furthermore its dysregulation can impact proteins such as ACC1 (acetyl-CoA carboxylase) in the context of metabolic syndrome. Research continues to explore the implications of CPT1A in non-alcoholic steatohepatitis highlighting its relevance in liver energy metabolism disorders and their pathophysiology.