Abcam, ab1287, Anti-Aurora A antibody - Centrosome Marker

Size: 50µg
Rabbit Polyclonal Aurora A antibody. Centrosome marker. Suitable for IP and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Synthetic Peptide within Human AURKA conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AURKA conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.O14965

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.1% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aurora A also known as Aurora kinase A is an important serine/threonine kinase with a protein mass of approximately 46 kDa. It is commonly found in various tissues but its expression is particularly high during mitosis in dividing cells. Aurora A operates primarily at the centrosomes where it is pivotal for processes such as centrosome maturation separation and the establishment of the mitotic spindle. The kinase activity of Aurora A is important for proper chromosome alignment and segregation. Scientists often use Aurora A as a marker in studies related to cell division and cancer.
Biological function summary
Aurora A plays key roles in the regulation of the cell cycle and mitosis. It functions as part of the Aurora mouse kinase complex associating with other proteins to ensure accurate chromosomal segregation and cytokinesis. Aurora A is involved in centrosome dynamics and interacts with proteins such as 35C1 aiding its localization to the correct cellular structures. The protein’s role in the cell cycle and mitotic checkpoints makes it a subject of interest for researchers examining cancer proliferation.
Pathways
Aurora A integrates into both the mitotic spindle assembly checkpoint and DNA damage repair pathways. Within these pathways Aurora A partners with proteins like APC (Anaphase Promoting Complex) to ensure timely progression through mitosis and safeguard genomic stability. The spindle assembly pathway ensures that spindle fibers correctly attach to chromosomes a process in which Aurora A proves instrumental. Its interaction with other kinases and regulatory molecules in these pathways highlights its regulatory importance in cell proliferation.
Research identifies Aurora A's association with oncological conditions such as breast and ovarian cancers. Overexpression or amplification of Aurora A correlates with tumor progression and poor prognosis as it can lead to chromosomal instability. In these diseases Aurora A activity is linked with dysregulated pathways involving proteins like p53 and BRCA1 which normally function in DNA damage response and repair. Targeted inhibition of Aurora A represents a promising avenue in cancer therapy as it might restore normal cell cycle control and reduce cancer cell viability.