Abcam, ab128874, Anti-Brd4 antibody [EPR5150(2)]

Size: 100µL / 1mL
Anti-Brd4 antibody [EPR5150(2)] (ab128874) is a rabbit monoclonal antibody detecting Brd4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 160 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5150(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:Flow Cyt (Intra), ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Brd4 antibody [EPR5150(2)] (ab128874) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-Brd4 antibody [EPR5150(2)] (ab128874) was first used in a scientific publication in 2014 and has been cited over 163 times in peer reviewed journals. It's performance in Western Blot in human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Brd4 antibody [EPR5150(2)] (ab128874) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Brd4 antibody [EPR5150(2)] (ab128874) has been confirmed by Western Blot testing in Brd4 knockout Hap1 cells.
Anti-Brd4 antibody [EPR5150(2)] (ab128874) has 17 independent reviews from customers.
Anti-Brd4 antibody [EPR5150(2)] (ab128874) specifically detects Brd4 (UniProt ID: O60885; Molecular weight: 153kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR5150(2) -
ab182446
Antibody clone EPR5150(2) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, HRP, Alexa Fluor
ab197606
ab197608
ab197609
ab207057
Top cited antibody for this target with >200 citations. A highly specific BRD4 antibody, essential for studying bromodomain-containing protein 4, epigenetic regulation and chromatin remodeling. This antibody is crucial in cancer research, particularly in understanding BRD4's role in transcription regulation, chemoresistance and tumor progression and is widely used in studies of BRD4 inhibitors and BET proteins. Research has shown that BRD4 (and BET family proteins) may be promising therapeutic targets for various Myc-driven cancers, such as Burkitt's lymphoma and certain acute myeloid leukaemia's and is studied is a potential therapeutic target.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Shipping conditions update: ambient shipping
This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.
Why the change?
It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.
What you need to know
Ambient shipments come clearly marked on the delivery note.
No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
Warranty coverage remains fully valid, aligned with our validated shipping method.
Please store the product as per the datasheet instructions upon receipt.
Find out more -
https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BRD4 also known as Bromodomain-containing protein 4 is a member of the BET (bromodomain and extra-terminal domain) family. This protein has a molecular weight of approximately 120 - 150 kDa often used in western blot analysis to identify its presence. It binds to acetylated lysines on histone proteins facilitating the regulation of gene expression. BRD4 is mainly expressed in the nucleus and plays an essential role in remodeling chromatin structure therefore influencing transcription processes.
Biological function summary
This protein influences various cellular activities by regulating transcriptional elongation of specific genes. BRD4 forms part of a complex with P-TEFb (Positive Transcription Elongation Factor b) which phosphorylates the C-terminal domain of RNA polymerase II facilitating transcription through chromatin. It binds to acetylated histones and non-histone proteins linking it to active transcription sites and contributing to the dynamic regulation of gene expression in response to cellular signals.
Pathways
BRD4 participates in key signaling networks like the NF-κB pathway and MYC signaling. It interacts with transcription factors and other proteins such as Cyclin T1 and CDK9 within the P-TEFb complex to regulate gene expression. BRD4 also collaborates with MYC a critical transcription factor regulating cell growth and proliferation to modulate pathways related to cellular growth and survival.
BRD4 has been linked to cancer and inflammation-related conditions. Overexpression or mutations in BRD4 can lead to aberrant cell proliferation in certain cancers including breast and leukemia where it influences the expression of oncogenes. In inflammation BRD4 modulates the expression of pro-inflammatory cytokines. Through these pathways it is connected to related proteins like NF-κB which orchestrates cellular responses to inflammation and stress signals.