Abcam, ab128901, Anti-WIPI1 antibody [EPR8110]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal WIPI1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8110,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WIPI1 also known as WD repeat domain phosphoinositide-interacting protein 1 is a protein involved in cellular processes especially autophagy. WIPI1 has a molecular mass of approximately 50 kDa and consists of WD40 repeats a common motif in proteins involved in signal transduction and cell cycle control. This protein is found in various human tissues with high expression in the heart skeletal muscle and pancreas indicating its involvement in essential metabolic and regulatory activities.
Biological function summary
WIPI1 has an important role in the process of autophagy acting as part of the PI3P-binding proteins family. It functions as a component of the autophagy initiation complex contributing to the formation of autophagosomes by recruiting additional autophagic proteins. WIPI1 interacts with ATG2 proteins facilitating the elongation of isolation membranes. Its activity is influenced by the availability of phosphatidylinositol 3-phosphate (PI3P) which is a critical lipid signaling molecule in cells.
Pathways
WIPI1 participates in the mechanistic target of rapamycin (mTOR) signaling pathway and the PI3K-Akt signaling pathway. In the mTOR pathway WIPI1 negatively regulates mTORC1 signaling therefore promoting autophagosome formation under nutrient-deprived conditions. This interaction positions WIPI1 as an inhibitor of mTORC1-mediated nutrient sensing. In collaboration with ULK1 and BECN1 WIPI1 supports autophagic flux by assisting in the early stages of autophagosome formation therefore contributing to cellular homeostasis.
Abnormalities in WIPI1 expression and function have links to cancer and neurodegenerative diseases. In certain types of cancer including breast cancer altered levels of WIPI1 correlate with tumor progression and poor prognosis potentially through interactions with mTOR. Additionally in neurodegenerative conditions like Alzheimer's disease dysregulated autophagy pathways involving WIPI1 and ATG proteins may lead to accumulation of damaged proteins and organelles accentuating disease symptoms.