Abcam, ab128996, Anti-eIF3A antibody [EPR5819(2)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal EIF3A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5819(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eukaryotic translation initiation factor 3 subunit A (eIF3A) sometimes known as eIF3 p170 is an important component in the initiation of protein synthesis. This protein with a molecular mass of approximately 170 kDa plays an important role in forming the eIF3 complex which is essential for the binding of mRNA to the ribosome. eIF3A is widely expressed in various tissues indicating its fundamental role in basic cellular functions.
Biological function summary
EIF3A is a central component of the eIF3 complex which consists of multiple subunits that interact to facilitate the beginning of translation. It supports the recruitment of mRNA to the small ribosomal subunit mediating the formation of the 43S pre-initiation complex. Its activity influences multiple downstream biological processes including cell growth and proliferation highlighting its essential role in maintaining normal cellular functions.
Pathways
EIF3A is integral to the mTOR signaling pathway which is important for regulating cell growth and protein synthesis. In this pathway eIF3A works closely with proteins like mTOR and Raptor interacting to control the translation initiation machinery. Additionally eIF3A contributes to the regulation of the Wnt signaling pathway which is important for cell proliferation and differentiation.
EIF3A has significant connections with various cancer types including lung cancer and breast cancer. Its overexpression often correlates with enhanced tumor growth and poor prognosis implicating its role in oncogenic pathways. In cancerous conditions eIF3A might interact with proteins such as EGFR and c-Myc which are also key players in tumor progression and cellular transformation further emphasizing its impact on disease development.