Abcam, ab131451, Anti-CPI-17 antibody

Size: 100µg
Rabbit Polyclonal CPI-17 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R14A aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PPP1R14A aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q96A00

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab131451 was purified by affinity chromatography using epitope specific peptide., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CPI-17 also known as Protein Kinase C-Potentiated Inhibitor Protein-17 is a protein that acts as a myosin phosphatase inhibitor. It has a molecular mass of approximately 17 kDa. Expressed in various tissues including smooth muscle heart and brain CPI-17 plays an essential role in regulating smooth muscle contractility. The protein modulates the phosphorylation state of myosin light chains by inhibiting myosin phosphatase activity impacting muscle contraction dynamics.
Biological function summary
CPI-17 influences smooth muscle function by modulating the phosphorylation status of myosin light chains. It is not a component of larger protein complexes but interacts directly with myosin phosphatase. The precise regulation of myosin light chain phosphorylation by CPI-17 is critical for maintaining smooth muscle contractile responses affecting functions such as vasoconstriction and peristalsis.
Pathways
CPI-17 plays an important role in the RhoA/ROCK signaling pathway which is key to controlling smooth muscle tension and motility. Through this pathway CPI-17 interacts closely with proteins like ROCK (Rho-associated coiled-coil containing protein kinase). Additionally CPI-17 is associated with the PKC (Protein Kinase C) pathway where its phosphorylation status and inhibitory activity are regulated by PKC enzymes emphasizing its role in cellular signal transduction.
CPI-17 is implicated in conditions such as hypertension and asthma where dysregulated smooth muscle contractility is a factor. In hypertension CPI-17's interaction with the PKC pathway suggests a substantial influence on blood vessel tension while in asthma altered CPI-17 activity affects airway smooth muscle contraction. The link between CPI-17 and PKC proteins under these conditions provides insight into potential therapeutic targets for managing these diseases.