Abcam, ab131499, Anti-JNK1 + JNK2 (phospho T183 + Y185) antibody

Size: 100µg
Rabbit Polyclonal JNK1 phospho Y185 + T183 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human MAPK8 phospho Y185 + T183.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Mouse,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MAPK8 phospho Y185 + T183. The exact immunogen used to generate this antibody is proprietary information.P45983,
Specificity:The region of JNK1 and JNK2 surrounding T183 + Y185 has a high degree of similarity to the corresponding regions in JNK3 and thus may cross react with this protein if phosphorylated on the corresponding residues.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This antibody was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
JNK1 and JNK2 are part of the c-Jun N-terminal kinase group also called Stress-activated protein kinases (SAPKs). These proteins are serine/threonine kinases with a known molecular weight around 46-54 kDa depending on their phosphorylation state. JNK1 and JNK2 phosphorylate specific target proteins which include transcription factors and other kinases to regulate various cellular processes. They are expressed in most tissues with higher levels in brain heart and skeletal muscle. JNK isoforms have become essential in research involving stress responses where specific tools such as JNK antibodies and Western blot assays targeting p-JNK molecular weight are critical for study.
Biological function summary
These enzymes play vital roles in regulating cellular responses to stress stimuli. JNK1 and JNK2 form part of larger signaling complexes including the JNK molecular weight complex that is activated in stressful conditions like UV radiation or cytokines. Once activated they phosphorylate transcription factors such as c-Jun leading to gene expression changes that facilitate adaptative and survival responses. They modulate cellular processes like apoptosis inflammation and cell differentiation clearly contributing to homeostasis and development.
Pathways
These kinases interact significantly within the MAPK (Mitogen-activated protein kinase) and apoptosis pathways. JNK proteins mediate signals from upstream kinases such as MKK4/7 and respond to inflammatory cytokines resulting in transcriptional alterations. Their downstream impact is tightly linked to apoptosis through interactions with Bcl-2 family members affecting cellular fate. ERK and p38 MAPK proteins closely interact with JNK pathways co-regulating cellular stress responses and survival pathways highlighting their importance in maintaining cellular balance.
The altered regulation of JNK1 and JNK2 links them to diverse conditions such as cancer and neurodegenerative diseases. In cancer abnormal JNK activity affects cell proliferation and apoptosis with JNK pathways often hyperactivated in tumors. In neurodegeneration these kinases contribute to cell death as seen in disorders like Alzheimer's disease where they engage with proteins like tau leading to neuronal apoptosis. The connection of JNK proteins to other stress-activated kinases further positions them as critical therapeutic targets in disease modulation.