Abcam, ab131556, Human TLR2 ELISA Kit (CD282)

Size: 1 x 96Tests
Human TLR2 ELISA Kit (CD282) is a Sandwich (qualitative) ELISA for the measurement of Human TLR2 (CD282) in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (qualitative),
Sensitivity:< 10 pg/mL,
Range:156 - 10000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's Human TLR2 (CD282)
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human soluble TLR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
A TLR2 (CD282) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for TLR2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TLR2 amount of sample captured in plate.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TLR2 also known as Toll-like receptor 2 is a type 1 transmembrane protein playing a role in the innate immune system. This protein has a molecular mass of approximately 90 kDa. It is present on the surface of immune cells such as macrophages dendritic cells and some types of epithelial cells. TLR2 recognizes and binds to pathogen-associated molecular patterns acting as a receptor for lipoproteins and lipopeptides from Gram-positive bacteria mycobacteria and fungi.
Biological function summary
TLR2 works as part of a complex with other proteins like TLR1 or TLR6 to initiate the immune response. It helps detect microbial components which leads to the activation of downstream signaling pathways and subsequent release of pro-inflammatory cytokines. TLR2 acts as an important mediator in recognizing a variety of pathogens facilitating an effective immune response.
Pathways
TLR2 is involved in key immune signaling pathways such as the NF-kB and MAPK pathways. These pathways lead to the transcription of genes important for inflammatory responses and production of cytokines and chemokines. TLR2 interacts with other proteins like MyD88 and IRAK which are critical in propagating the signal that results in an inflammatory response tailored to combating the specific invading pathogens.
TLR2 plays a significant role in conditions like rheumatoid arthritis and sepsis. Its overactivation can lead to excessive inflammatory responses contributing to the pathogenesis of these diseases. The protein is often in connection with other receptors like TLR4 which together can amplify inflammation exacerbating disease symptoms. Understanding TLR2's role in these processes can provide insights into therapeutic targets for managing inflammatory disorders.