Abcam, ab131665, Recombinant human Heme-regulated inhibitor protein

Size: 10µg
Recombinant human Heme-regulated inhibitor protein is a Human Full Length protein, in the 1 to 630 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab131665 was determined to be 12 nmol/min/mg.,
Accession:Q9BQI3,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
ab204878
(RS Repeat Peptide peptide) can be utilized as a substrate for assessing kinase activity
Previously labelled as EIF2AK1.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heme-regulated inhibitor (HRI) also known as eIF2α kinase 1 plays an important role in controlling protein synthesis in response to heme availability. It weighs approximately 80 kDa and is primarily expressed in erythroid cells in the bone marrow where hemoglobin synthesis occurs. HRI works as a sensor of heme levels and regulates the translation initiation process by phosphorylating the eukaryotic translation initiation factor 2 alpha (eIF2α) which reduces general protein synthesis under heme-deficient conditions.
Biological function summary
Heme-regulated inhibitor functions as an important part of the cellular stress response. It is not a standalone entity and often forms part of larger signaling networks as it interacts with eIF2α. In addition to its role in erythroid cells HRI responds to various stressors including oxidative stress and heat shock by modulating protein production which contributes to cellular adaptation mechanisms. Its activity helps maintain homeostasis by reducing protein synthesis when cells face adverse conditions.
Pathways
HRI significantly influences the integrated stress response (ISR) pathway and iron metabolism. HRI's activation through phosphorylation of eIF2α links it to related proteins such as activating transcription factor 4 (ATF4) which assists in adaptive gene expression changes under stress conditions. This action highlights HRI's involvement in pathways that regulate translation under various stress conditions maintaining proper cellular function and response to changes in environmental and internal conditions.
HRI has a pivotal involvement in conditions like anemia and beta-thalassemia. An imbalance in HRI activity can lead to inadequate or excessive protein synthesis contributing to disease states. It connects to alpha-globin and beta-globin proteins through its regulation of hemoglobin synthesis in erythroid cells. In iron deficiency anemia HRI activation prevents excess protein synthesis that would otherwise require unavailable heme while its dysfunction might exacerbate issues in beta-thalassemia by impairing stress response and erythropoiesis.