Abcam, ab131670, Recombinant Human XLF protein

Size: 20µg
Recombinant Human XLF protein is a Human Fragment protein, in the 1 to 224 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9H9Q4,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The XLF protein also known as XRCC4-like factor plays an important role in DNA repair mechanisms. It weighs approximately 33 kDa and is expressed in various tissues with high levels noted in the thymus and testis. XLF functions as a part of the non-homologous end joining (NHEJ) pathway where it aids in the repair of DNA double-strand breaks. It acts by promoting the ligation of broken DNA ends often working closely with other proteins such as XRCC4 and DNA ligase IV.
Biological function summary
XLF participates in the critical process of maintaining genomic stability. It is part of the NHEJ complex alongside XRCC4 and DNA ligase IV ensuring efficient repair of DNA breaks. This repair process is essential for preventing genomic instability and protecting cells from deleterious mutations. XLF helps tether DNA ends together providing structural support necessary for effective repair and its function is vital for cell survival especially in rapidly dividing cells.
Pathways
This protein integrates into the NHEJ pathway a primary mechanism for repairing DNA double-strand breaks. XLF collaborates directly with other proteins like Ku70/80 and DNA-PKcs facilitating their functions in the DNA damage response. Additionally it plays a role in the V(D)J recombination process which is vital for the development of immune system diversity by allowing the rearrangement of variable (V) diversity (D) and joining (J) gene segments.
XLF relates to severe combined immunodeficiency (SCID) and neurodegenerative disorders. XLF deficiencies can manifest as immunodeficiency due to impaired V(D)J recombination adversely affecting the immune response. It is also linked to neurodegeneration because proper DNA repair is important for neuronal survival. Other proteins in these contexts include DNA ligase IV which shows connection in supporting similar cellular functions and diseases.