Abcam, ab131686, Recombinant Human THG1L protein

Size: 20µg
Recombinant Human THG1L protein is a Human Fragment protein, in the 30 to 298 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9NWX6,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
THG1L also known as tRNA-histidine guanylyltransferase 1-like is an enzyme responsible for adding a guanine nucleotide to the 5' end of tRNA(His). This enzyme weighs approximately 48 kDa and is encoded by the THG1L gene. THG1L is highly conserved across eukaryotes and its expression is mostly observed in the mitochondria and cytosol of human cells. The protein ensures the proper modification and functioning of tRNA which is important for accurate protein synthesis.
Biological function summary
TRNA processing involves an important step facilitated by THG1L. This enzyme is important for maintaining the integrity of tRNA(His) by forming a specific guanine nucleotide cap at its 5' end. THG1L does not belong to any known large protein complex but works in coordination with other enzymes in the tRNA maturation process. It ensures the appropriate modifications in tRNAs which are essential for their correct recognition by ribosomes during translation.
Pathways
THG1L plays an essential role in the tRNA modification pathway particularly in histidine tRNA processing. The presence of THG1L facilitates the correct attachment of histidine to its tRNA therefore maintaining the fidelity of the translation process. This enzyme interacts with other proteins involved in the tRNA aminoacylation pathway including aminoacyl-tRNA synthetases which are important for the attachment of amino acids to their corresponding tRNAs during protein synthesis.
Researchers have investigated THG1L in the context of mitochondrial diseases and neurodegenerative disorders. Abnormalities in THG1L function can lead to improper tRNA processing which may result in mitochondrial dysfunction or contribute to the pathogenesis of neurological disorders. The interaction of THG1L with mitochondrial proteins such as those involved in oxidative phosphorylation suggests a potential link between mutations in THG1L and mitochondrial-related diseases.