Abcam, ab132100, Recombinant Human N-ethylmaleimide-sensitive fusion protein

Size: 10µg
Recombinant Human N-ethylmaleimide-sensitive fusion protein is a Human Full Length protein, in the 1 to 744 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P46459,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as NSF.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N-ethylmaleimide-sensitive fusion protein (NSF) is an important component in the cellular machinery. It unwinds homo-oligomer complexes enabling membrane fusion in cells. NSF also goes by the name of NEM-sensitive factor. It has a mass of about 82 kDa. It is expressed in most tissues where membrane fusion processes occur such as neurons liver and other secretory cells.
Biological function summary
NSF plays a central role in ensuring proper vesicular transport. It is part of the SNARE complex which facilitates the fusion of vesicles with their target membranes. Interaction with small regulatory proteins like SNAPs (Soluble NSF Attachment Proteins) ensures NSF activity in recycling SNARE proteins after vesicle fusion. This process is critical for neurotransmitter release in neuronal synapses and for enzymatic secretion in glandular tissues.
Pathways
NSF contributes significantly to intracellular vesicle trafficking and neurotransmitter release pathways. In these pathways proteins like VAMP synaptobrevin and SNAP-25 interact with NSF to maintain the continuity of vesicle-mediated transport. NSF activity modulates SNARE-mediated vesicle fusion thereby allowing efficient cargo transfer between cellular compartments.
NSF has connections to neurological disorders and certain protein trafficking diseases. Mutations in NSF or dysfunction of its SNARE partners can link to neurodegenerative diseases like Alzheimer’s disease. Proteins like the amyloid-beta precursor protein can also interact aberrantly with NSF pathway components exacerbating disease states. Additionally improper NSF function may relate to cystic fibrosis due to issues in vesicular transport affecting CFTR protein localization.