Abcam, ab132115, Recombinant Human PGE2 receptor EP4 subtype protein

Size: 2µg / 10µg
Recombinant Human PGE2 receptor EP4 subtype protein is a Human Full Length protein, in the 1 to 488 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P35408,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as Prostaglandin E Receptor EP4.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PGE2 receptor EP4 subtype also known as PTGER4 is a member of the G protein-coupled receptor (GPCR) family. This receptor binds prostaglandin E2 and plays a role in various physiological processes. The protein has an approximate mass of 65 kDa. EP4 receptor is widely expressed in tissues such as the immune system bone gastrointestinal tract and reproductive organs indicating its diverse functional implications. Additionally EP4 receptor is activated by PGE2 EP4 agonists which help in modulating its activity.
Biological function summary
EP4 receptor influences numerous functions including inflammation immune response and bone metabolism. The receptor is not part of a larger complex but interacts with different signaling molecules and secondary messengers during its activity. It modulates responses mainly through cAMP which further amplifies or reduces cellular responses depending on the context. These biological roles display the versatile effects EP4 can exert during various physiological scenarios.
Pathways
The EP4 receptor is a significant component in the prostaglandin signaling pathway. This pathway involves the conversion of arachidonic acid to prostaglandin E2 followed by binding to EP4 triggering downstream effects via cAMP-related signals. Within this pathway it interacts closely with proteins like adenylate cyclase which plays a role in regulating cAMP levels. EP4 also participates in the Wnt signaling pathway where it can either enhance or inhibit processes by interacting with other signaling cascade proteins.
EP4 receptor has connections with conditions like rheumatoid arthritis and bone-related diseases. In rheumatoid arthritis excessive signaling through EP4 exacerbates inflammatory responses in joint tissue. The receptor potentially associates with inflammatory cytokines like tumor necrosis factor-alpha (TNF-alpha) contributing to disease severity. In bone-related diseases such as osteoporosis EP4 influences bone remodeling and homeostasis linking to proteins involved in bone formation and resorption dynamics.