Abcam, ab132258, Recombinant Human SPAM1 protein

Size: 2µg
Recombinant Human SPAM1 protein is a Human Full Length protein, in the 1 to 509 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P38567,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as Hyaluronidase PH20.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPAM1 also known as sperm adhesion molecule 1 or PH-20 refers to a glycoprotein found on the sperm cell surface. Its molecular mass is approximately 64 kDa. This protein is primarily expressed in the testes and epididymis where it plays a role in sperm maturation. SPAM1 possesses hyaluronidase activity breaking down hyaluronic acid present in the cumulus cell matrix of the female reproductive tract during fertilization. It aids sperm in penetrating the egg's outer layers which is important for successful fertilization.
Biological function summary
SPAM1 participates in sperm penetration through the zona pellucida an essential step in fertilization. It acts independently facilitating sperm passage by degrading surrounding extracellular matrices. SPAM1 while not part of a larger protein complex interacts directly with the female reproductive tract's biochemical structures. These interactions ensure an efficient sperm-vs-egg recognition process that aids in sperm-egg fusion. Its enzymatic activity is central to this sperm-egg binding and membrane fusion.
Pathways
SPAM1 contributes significantly to the fertilization pathway specifically the Acrosome Reaction. It interacts indirectly with proteins like ZP3 a zona pellucida protein that triggers the acrosome reaction necessary for sperm to penetrate the ovum. Additionally SPAM1 plays a minor role in the hyaluronan-mediated motility pathway interacting faintly with the extracellular matrix components to enhance sperm motility and penetration.
SPAM1 has been implicated in certain infertility cases particularly those involving the failure of sperm to penetrate the egg successfully. This condition might relate to defects or alterations in SPAM1 activity or expression. Moreover some studies suggest a possible link between SPAM1 and testicular cancer where aberrant expression could influence tumorigenesis. SPAM1 anomalies may associate with other proteins like EZH2 involved in various cancers suggesting broader functional implications beyond reproductive biology.