Abcam, ab132965, Recombinant Human APOBEC3G/A3G protein

Size: 10µg
Recombinant Human APOBEC3G/A3G protein is a Human Full Length protein, in the 1 to 384 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9HC16,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as APOBEC3G.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APOBEC3G also known as A3G is an enzyme that belongs to the APOBEC family of cytidine deaminases. It has a molecular mass of approximately 46 kDa. This protein is expressed in various tissues including immune cells like lymphocytes and macrophages. A3G plays a mechanical role in catalyzing the deamination of cytidine to uridine on single-stranded DNA which impacts the genetic material of retroviruses.
Biological function summary
APOBEC3G acts as a host defense mechanism against retroviral infections particularly HIV-1. It incorporates into budding virions where it exerts its antiviral effect. A3G functions within the APOBEC protein family complex that participates in innate immunity. This antiviral activity helps to inhibit viral replication by inducing lethal mutations in the viral genome.
Pathways
APOBEC3G is vital in the HIV-1 life cycle disruption pathway. It interacts with other cellular factors such as the viral protein Vif which counters APOBEC3G’s activity by targeting it for proteasomal degradation. The protein is also involved in the regulation of gene expression pathways linked to innate immune responses.
APOBEC3G is associated with HIV-1 infection. Elevated levels of APOBEC3G can lead to the hypermutation of viral DNA potentially reducing viral loads in host cells. The protein’s interaction with HIV-1 Vif protein is critical as Vif facilitates the degradation of A3G allowing the virus to escape its antiviral activity. Additionally researchers investigate a possible connection between A3G and cancer due to its mutation-inducing capability in DNA.