Abcam, ab133111, SREBP-2 Transcription Factor Assay Kit

Size: 96Test
Abcam's SREBP-2 Transcription Factor Assay Kit (ab133111) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
Key facts
Detection method:Colorimetric,
Sample types:Nuclear Extracts, Suspension cells, Adherent cells,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Microplate reader

Product details:
Abcam's SREBP-2 Transcription Factor Assay Kit (ab133111) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized to the wells of a 96-well plate. SREBP contained in a nuclear extract, binds specifically to the SREBP response element. SREBP is detected by addition of a specific primary antibody directed against SREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory elements binding proteins (SREBPs). SREBPs directly activate the expression of over 30 gened involved in teh synthesis and uptake of cholesterol, fatty cids, triglycerides and phopholipids. SREBP-2 activates cholesterol synthesis by upregulating expression of HMG-CoA reductase.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols